1. 제품 특징
- · MMLV reverse transcriptase (RTase), ribonuclease inhibitor, dNTPs 그리고 oligo(dT)s와 random primers가 최적의 비율로 구성
- · 신속하고 정확하게 cDNA를 합성 (20분 이내)
- · 사용하기 편리한 Master Mix 형태 제품
- · cDNA synthesis reaction volume: 10 ul (CDS 제품), 20 ul (CDSP 제품)
2. 제품 결과
Figure. qPCR Result of GAPDH gene (148bp fragment) of human cDNA made using CellScript 5X cDNA Synthesis Master Mix. Reactions were carried out on the same plate using CFX96 on cDNA inputs of 100ng, 10ng, 1ng, 100pg, 10pg respectively. The entire plate was activated at 95ºC for 1minutes. The cycling was carried out 40cycles, 95ºC (5sec), 60ºC (10sec) and 72ºC (15sec).
3. 제품 종류