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DNA free Taq DNA Polymerase


제품비교

50,000~450,000

클리어

대부분의 Taq DNA polymerase는 E.coli DNA가 포함되어 아래와 같은 문제점 발생

  • 비특이적인 side products 생성
  • Primer-dimer 형성 촉진
  • Multiplex PCR에서 false positive 결과 유도
  • qPCR 반응시 Ct값 limit 제한 – 민감도 저하
  • 유전자 진단의 적용 제한
  • Cloning시 잘못된 유전자 획득
  • Sequencing시 염기해독의 오류

1. 제품 특징

  • Source : Thermus aquaticus
  • 5’→3’ exonuclease activity : Yes
  • 3’→5’ exonuclease activity : No
  • Amplification size : <3kb
  • A-tailing : Yes

 

2. 타세 제품 비교 결과

PCR was performed without template with E. coli 16s rDNA specific primers (1.2 kb product).
MW – 100bp plus

  • 2.5unit DNA free-Taq of CellSafe (30cycles)
  • 1.25 unit DNA free-Taq of CellSafe (30cycles)
  • 1.25 unit Taq of competitor A
  • 1.25 unit Taq of competitor B
  • 1.25 unit Taq of competitor C
  • 1.25 unit Taq of competitor D

3. 제품 적용

  • Routine PCR, multiplex PCR and qPCR
  • Allele specific PCR
  • 16S and 23S rRNA gene amplification
  • Detection of bacteria in samples (e.g. blood)
  • DNA labeling reactions & TA-cloning
  • Sequencing/ cycle sequencing

 

4. 제품 종류

 
Size

500units, 1,000units, 2,500units, 5mL, 96(8strip x 12ea), 480(8strip x 60ea)

Type

Enzyme, Master Mix, Premix

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